Reverse phase-high performance liquid chromatography (RP-HPLC) method have been developed and validated for the estimation of edaravone and citicoline sodium in bulk drug and synthetic mixture. The developed method is rapid, accurate, precise, simple and economical. The separation was carried out using luna C18 100A° (250 mm×4.6 mm i.d.) 5 µm reverse phase column (phenomenex, luna®) in gradient mode, with mobile phase containing Acetonitrile: Water (70:30, v/v). The flow rate is 0.8 ml/min and effluents are monitored at 244 nm. Chromatogram showed peak at a retention time of 3.667±0.008 min for edaravone and 2.258±0.008 min for citicoline sodium. The method is validated for system suitability, linearity, precision, accuracy specificity, ruggedness, robustness, LOD and LOQ. Recovery of edaravone and citicoline sodium is found to be in the range of 99.94 - 100.19 %. The LOD and LOQ for estimation of edaravone and citicoline sodium are found to be 0.078 µg/ml, 0.237µg/ml and 1.223µg/ml, 3.707 µg/ml respectively. Proposed method can be successfully applied for the quantitative determination of edaravone and citicoline sodium in bulk drug and in synthetic mixture.
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